| 07 Charlotte Independence ECRL South G | Charlotte Independence SC (CISC) | NC |
| CISC 07 G ECNL Futures North | Charlotte Independence SC (CISC) | NC |
| FC Alliance 07 Girls ECNL | FC Alliance (TN) | TN |
| FC Alliance 08 Girls ECNL | FC Alliance (TN) | TN |
| Indiana Fire Juniors South 07G Red | Indiana Fire Juniors | IN |
| Knoxville Reds 08 Newcastle | Knoxville Reds FC | TN |
| CISC 07 G Select | Charlotte Independence SC (CISC) | NC |
| Commonwealth SC 2007 Girls Black | Commonwealth Soccer Club | KY |
| FC Alliance 2007G ECNL-RL | FC Alliance (TN) | TN |
| Kolping 07g Orange | Kolping SC | OH |
| 07 (15U) LYSA Force G | Lenoir Youth Soccer (LYSA) | NC |
| Commonwealth SC 2008 Girls Black | Commonwealth Soccer Club | KY |
| Indiana Impact 07 Girls Elite | Indiana Impact SC | IN |
| LFC 07G Red | Lexington FC | KY |
| 06 (16U) HFC Red G | Highland Football Club (HFC) | NC |
| 06 Charlotte Independence ECRL South G | Charlotte Independence SC (CISC) | NC |
| CISC 06 G ECNL Futures North | Charlotte Independence SC (CISC) | NC |
| Sporting Columbus 2006 | Sporting Columbus | OH |
| TUSC 06G SCCL | Tennessee United Soccer Club | TN |
| CISC 06 G Select | Charlotte Independence SC (CISC) | NC |
| Cleveland Force 06G Blue | Cleveland Force Soccer Club | OH |
| DFA 2006 Girls Premier | Dayton Futbol Academy (DFA) | OH |
| FC Alliance 06g ECNL-RL | FC Alliance (TN) | TN |
| Impact 06 Girls Elite | Indiana Impact SC | IN |
| Tri-Cities United 06/07 Girls Premier | Tri-Cities United Soccer Club | TN |
| Commonwealth SC 2005 Girls Gold | Commonwealth Soccer Club | KY |
| CSC 05 Girls | Clarksville Soccer Club (TN) | TN |
| DFA Girls 05 Premier | Dayton Futbol Academy (DFA) | OH |
| FC Alliance 05G ECNL-RL | FC Alliance (TN) | TN |
| KHA North G05 Red | Kings Hammer Academy (OH) | OH |
| Polaris SC Navy 05G | Polaris Soccer Club | OH |
| USA Southeast 05/04G Premier | USA Southeast | TN |
| 04 (17U) CISC North NM Blue G | Charlotte Independence SC (CISC) | NC |
| FC Alliance ECNL-RL GU19 | FC Alliance (TN) | TN |
| Knoxville Reds 04G United | Knoxville Reds FC | TN |
| Kolping SC G04 Orange | Kolping SC | OH |
| Polaris SC Navy 04G | Polaris Soccer Club | OH |
| Tri-Cities United 04/05 Girls Premier | Tri-Cities United Soccer Club | TN |
| 04 (18U) HFC Red G | Highland Football Club (HFC) | NC |
| CASA Pride 04 Girls Premier | CASA Pride - Clemson Anderson SA | SC |
| Dayton Futbol Academy DFA Elite G04 | Dayton Futbol Academy (DFA) | OH |
| Warrior 04 Premier | Warrior Soccer Club (OH) | OH |
| Warrior 04G Elite | Warrior Soccer Club (OH) | OH |
| Alliance Cincinnati SC Elite | Alliance Cincinnati | OH |
| FC Alliance 04 ECNL | FC Alliance (TN) | TN |
| KHB 03G Premier | Kings Hammer Bluegrass | KY |
| Sporting Columbus 2003 | Sporting Columbus | OH |
| Clarksville SC 03/04 Maroon | Clarksville Soccer Club (TN) | TN |
| Commonwealth SC U19 Women | Commonwealth Soccer Club | KY |
| Nomads 03/04 Girls | Nomads United Soccer Club | TN |
| Park Athletic | UKSoccer Academy | KY |
| USA North 2004 Girls | USA North | TN |