| DPA Cobras 06 Girls Showcase | Dayton Players Academy | OH |
| FC Alliance 06 | FC Alliance (TN) | TN |
| Red Wolves Academy 06G Pool | Chattanooga Red Wolves Academy | TN |
| TUSC 06G Red | Tennessee United Soccer Club | TN |
| BSA Celtic G07 White | BSA Celtics | OH |
| CDA 06 Girls | Charlotte Development Academy | NC |
| Chattanooga FC 06 Navy | Chattanooga FC Academy | TN |
| FC Alliance 07 Girls | FC Alliance (TN) | TN |
| CDA 07 Girls | Charlotte Development Academy | NC |
| FC Alliance 06 SCCL | FC Alliance (TN) | TN |
| Metro FC 06 Rapids | Metro FC | OH |
| NSA Belles 15 | Northwest Soccer Academy | GA |
| CISC 05 G ECNL Futures North | Charlotte Independence SC (CISC) | NC |
| DPA Cobras 05 Girls Showcase | Dayton Players Academy | OH |
| Eastern Elite 05 Girls | Eastern Elite Soccer Accademy | KY |
| FC Alliance 05 ECNL | FC Alliance (TN) | TN |
| NUSA 05G Maroon | Nashville United Soccer Acd (NUSA) | TN |
| KHB 05G Premier | Kings Hammer Bluegrass | KY |
| Metro FC 05 Rapids | Metro FC | OH |
| NSA Belles 16 (TN) | Northwest Soccer Academy | GA |
| TSC Murfreesboro 05G Showcase | Tennessee Soccer Club (TN) | TN |
| 2005 Girls Elite | Metro East Legacy FC (IL) | IL |
| Barca Academy Columbus Red 05G | Barca Academy Columbus | OH |
| Dayton Futbol Academy Dfa 05g Elite | Dayton Futbol Academy (DFA) | OH |
| DPA Cobras 05 Girls Elite | Dayton Players Academy | OH |
| FC Alliance 05G SCCL | FC Alliance (TN) | TN |
| KHB G05 Classic | Kings Hammer Bluegrass | KY |
| 04 Lady Lobos Rush Premier | Lobos Rush Soccer | TN |
| CISC 04 G ECNL Futures North | Charlotte Independence SC (CISC) | NC |
| FC Alliance 04 | FC Alliance (TN) | TN |
| Knoxville Reds 04G United | Knoxville Reds FC | TN |
| USA Southeast 04 Girls Premier | USA Southeast | TN |
| Warrior 04G Elite | Warrior Soccer Club (OH) | OH |
| 04 Lady Lobos Rush Elite | Lobos Rush Soccer | TN |
| 04 WFC NPL G | Wake Futbol Club | NC |
| Barca Academy Columbus 04G Blue | Barca Academy Columbus | OH |
| Force 04/05 | Tinley-Frankfort (TFSC) | IL |
| NUSA 04G Maroon | Nashville United Soccer Acd (NUSA) | TN |
| Tri-Cities United 04/05 Girls Premier | Tri-Cities United Soccer Club | TN |
| 04/05 Select | Lobos Rush Soccer | TN |
| CISC NORTH LKN BLUE G | Charlotte Independence SC (CISC) | NC |
| CSC 04 Girls Maroon | Clarksville Soccer Club (TN) | TN |
| DPA Cobras 04-05 Elite | Dayton Players Academy | OH |
| KHB 04G Premier | Kings Hammer Bluegrass | KY |
| Warrior 04 Premier | Warrior Soccer Club (OH) | OH |
| CISC 02/03 NORTH LKN BLUE G | Charlotte Independence SC (CISC) | NC |
| Cleveland FC 03 | Cleveland Futbol Club | OH |
| Knoxville Reds 03G Santos | Knoxville Reds FC | TN |
| TUSC 2003 Girls Red | Tennessee United Soccer Club | TN |
| Knoxville Reds 03 Atletico | Knoxville Reds FC | TN |
| Nationals Genesee 03G Black | Nationals Soccer Club | MI |
| Nomads 2003/2004 | Nomads United Soccer Club | TN |
| WCSA G03 Premier | Warren County SA | OH |
| CISC 02 G ECNL Futures South | Charlotte Independence (SC) CISC-SC | SC |
| CISC 02/03 NM Blue G | Charlotte Independence SC (CISC) | NC |
| FC Alliance 03G | FC Alliance (TN) | TN |
| Knoxville Reds 02G Chivas | Knoxville Reds FC | TN |
| NUSA 02G Maroon | Nashville United Soccer Acd (NUSA) | TN |
| Tri-Cities United 02/03G Premier | Tri-Cities United Soccer Club | TN |
| KFC 02/03G Premier | Knoxville Football Club (TN) | TN |
| KHB 02G Premier | Kings Hammer Bluegrass | KY |
| MSC 02/03 Lady Strikers Premier | Murfreesboro Soccer Club | TN |
| NSA Belles 18/19 | Northwest Soccer Academy | GA |
| 02 HFC Red G | Highland Football Club (HFC) | NC |
| 02 Southeast Premier | USA Southeast | TN |
| FC Alliance 02\03G SCCL | FC Alliance (TN) | TN |
| Metro FC G02/03 Rapids | Metro FC | OH |
| Park Athletic | UK Soccer Academy | KY |
| YMCA Arsenal Rome 02 Girls | YMCA Arsenal Rome Soccer Club | GA |