| Participating Team National History |
| Age | Team | Championship Results |
| U20M | Cleveland United White | USYS 2025 Ohio State Semi-Finalist
USYS 2025 Ohio State Semi-Finalist
|
| Ohio Galaxies FC 96 Elite | USYS 2024 Ohio State Finalist
|
| Westside United Green | USYS 2025 Indiana State Champion
USYS 2024 Regional Semi-Finalist
|
| Galaxy 96/97 Blue | USYS 2016 Illinois State Semi-Finalist
US Club 2024 Regional Finalist
|
| Canton Celtic 98 Black | USYS 2025 Michigan State Quarter-Finalist
USYS 2025 Michigan State Quarter-Finalist
|
| Ohio Galaxies FC 97 Elite | USYS 2016 Ohio State Champion
USYS 2016 Ohio State Champion
|
| Westside United FC 98 Green | USYS 2016 Indiana State Finalist
|
| Warren County United Elite B97 | USYS 2025 Ohio State Semi-Finalist
|
| Ohio Galaxies FC 98 Elite | USYS 2017 Ohio State Semi-Finalist
USYS 2016 Ohio State Champion
|
| Ambassadors FC | USYS 2016 Ohio State Semi-Finalist
|
| North Shore United U16 Blue | USYS 2016 Wisconsin State Semi-Finalist
USYS 2016 Wisconsin State Semi-Finalist
|
| Ohio Galaxies FC 99 Premier Black | USYS 2016 Ohio State Semi-Finalist
|
| CKSC Kickers | USYS 2025 Ohio State Quarter-Finalist
|
| Ohio Galaxies FC 99 Elite | USYS 2018 Ohio State Finalist
|
| Madison FC Gold | USYS 2016 Wisconsin State Semi-Finalist
|