| Participating Team National History |
| Age | Team | Championship Results |
| U20F | Port Orange Hurricanes | USYS 2025 Florida State Quarter-Finalist
|
| Chiefs FC Elite 97 | USYS 2025 Georgia State Finalist
|
| AFC Lightning '97 Elite | USYS 2025 Regional Competitor
|
| Lady Phantoms Blue 98 | USYS 2025 Alabama State Finalist
|
| West KY PAO RDA | USYS 2024 Kentucky State Finalist
|
| AFC Lightning '98 Girls Elite | USYS 2016 Georgia State Finalist
USYS 2025 Georgia State Semi-Finalist
|
| VHSC Attack 99 Black II | USYS 2016 Alabama State Finalist
USYS 2025 Alabama State Semi-Finalist
|
| AFC Lightning '99 Girls Elite | USYS 2017 Georgia State Finalist
|
| Concorde Fire South Black | USYS 2017 Georgia State Semi-Finalist
USYS 2016 Georgia State Semi-Finalist
|
| IMG Academy U16 Girls | US Club 2024 National Competitor
|
| Pinecrest Premier Girls U-16 White | USYS 2025 Florida State Quarter-Finalist
|
| BUSA '00 Elite | USYS 2016 Alabama State Semi-Finalist
|
| LFC White 00 | USYS 2017 Kentucky State Finalist
USYS 2016 Kentucky State Finalist
USYS 2025 Regional Competitor
|
| 00 Lady Lobos Rush Premier | USYS 2018 Regional Competitor
|
| CUP Black G99 | USYS 2017 Ohio State Semi-Finalist
|
| SSA Chelsea 00G Elite | USYS 2025 Georgia State Semi-Finalist
|
| IMG Academy U15 Girls | USYS 2025 Florida State Semi-Finalist
|